6xHIS tag and immunofluorescence

[Oddmund Nordgård]

Hello!

I`m planning to make a 6xHis tagged fusion protein. Afterwards I want to use the

tag as an epitope for immunofluorescence. Has anybody tried this? Which antibody

do you suggest?

Have anybody tried the 6xHis antibody from Clontech? Or the one from Amersham

Pharmacia Biotech? Dianova? Qiagen?

What is your experience with expression of His-tagged proteins in mammalian cell

systems?

Thank you very much for any help!

Oddmund

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  Oddmund Nordgård
  Ph.D. student
  Institute of Biochemistry
  Box 1041 Blindern
  0316 OSLO
  NORWAY
  Phone: 22 85 66 99
  Fax:   22 85 44 43
  Email: oddmundn at biokjemi.uio.no

  Private:
  Kalbakkv. 21
  0953 OSLO
  Phone: 22 25 23 93

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