Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Fri Mar 3 05:17:57 EST 2000
In article <38C049FB.3207 at iris.sipp.ac.cn>, chenggy
<chenggy at iris.sipp.ac.cn> writes
>In column work,
> starting and washing buffer : 50 mM Tris-Cl buffer containing
> 1.7 M (NH4)2SO4
>eluted with gradient (NH4)2SO4 from 1.7 M to 0 M.
>Where is the enzyme? How can I found the reson?
Still stuck on the column? Try adding in 20% ethylene glycol to your
buffers or drop down to zero salt and then elute with an increasing urea
gradient i.e. 0 - 4M in Tris buffer plus 20% ethylene glycol.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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