Freezing GSH-agarose for "pull-down fever (PDF)"

Dr. Peter Gegenheimer PGegen at UKans.nolospamare.edu
Sat Mar 4 00:07:16 EST 2000


On Thu, 2 Mar 2000 01:10:04, Fred <dynamin at hotmail.com> wrote:

ð We attach GST-pusion proteins to GSH-agarose, and need to keep the beads
ð for a long time for a frenzy of pull-downs we are doing.  We find they
ð get bacterial contamination or lose activity after a month or so at
ð 4degC.  

Some beads -- I think including agarose -- are shipped in 20% EtOH. Unless 
this would kill the glutathione, you could store the beads at 4deg in 20% 
EtOH. 


o----------------------------------------------------------------------o
| Dr. Peter Gegenheimer       | Vox: 785-864-3939  FAX: 785-864-5321   |
| Department of               |   PGegen at UKans.nospam.edu              |
|   Molecular Biosciences     |   http://rnaworld.bio.ukans.edu/       |
| University of Kansas        |"When you have excluded the impossible, |
| 2045 Haworth Hall           |  whatever remains, however improbable, |
| Lawrence  KS  66045-2106    |  must be the truth."      S. Holmes    |
o_____________________________|________________________________________o





More information about the Proteins mailing list