Freezing GSH-agarose for "pull-down fever (PDF)"

Dr. Peter Gegenheimer PGegen at
Sat Mar 4 00:07:16 EST 2000

On Thu, 2 Mar 2000 01:10:04, Fred <dynamin at> wrote:

ð We attach GST-pusion proteins to GSH-agarose, and need to keep the beads
ð for a long time for a frenzy of pull-downs we are doing.  We find they
ð get bacterial contamination or lose activity after a month or so at
ð 4degC.  

Some beads -- I think including agarose -- are shipped in 20% EtOH. Unless 
this would kill the glutathione, you could store the beads at 4deg in 20% 

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