Freezing GSH-agarose for "pull-down fever (PDF)"

Dr. Peter Gegenheimer PGegen at UKans.nolospamare.edu
Mon Mar 6 18:13:18 EST 2000


On Sat, 4 Mar 2000 23:40:20, Anton Tutter <atutter at aim.salk.edu> wrote:

ð 
ð > Ð We attach GST-pusion proteins to GSH-agarose, and need to keep the beads
ð > Ð for a long time for a frenzy of pull-downs we are doing.  We find they
ð > Ð get bacterial contamination or lose activity after a month or so at
ð > Ð 4degC.
ð 
ð  
ð > Some beads -- I think including agarose -- are shipped in 20% EtOH. Unless
ð > this would kill the glutathione, you could store the beads at 4deg in 20%
ð > EtOH.
ð > 
ð 
ð i wouldn't do that if i were you!!!  i think peter misunderstood the
ð questions -- the beads are to be stored long-term *after* the GST-fusion
ð proteins are bound to the beads.  

Oops! I did misunderstand! Anton is quite right. In response to other 
questions, the presence of glycerol helps prevent ice crystal formation 
(that's why it's used for freezing bacterial cells). 

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