Richard P. Grant
rgrant at netscape.net
Mon Mar 13 07:18:56 EST 2000
In article <109ec386.d1a7c612 at usw-ex0109-066.remarq.com>, David Coulson
<d.coulsonNOd.SPAM at qub.ac.uk.invalid> wrote:
> I was wondering if anybody could help me with a "recipe" for
> making up Collodial Blue (Comassie G-250) solution for
> staining SDS-PAGE gels. Do you just make it up in water?,
> and if so what sort of concentration?
Coomassie brilliant blue R250? There is something which sounds similar
but doesn't work. Can't remember what it is, exactly.
Anyhoo: 0.1 % coomassie in 40% methanol, 10 % glacial acetic acid in
water (e.g. 2g dye, 800 ml MeOH, 200 ml GAA, make up to 2 l). Store at
4C and can be reused several times. Stain for about 15 - 20 mins at RT.
For destaining, I pour off the dye, then wash in dH20 (we have that on
tap) twice, then wash in destain (same as above, minus the dye) with
foam bungs to soak up the blue. This can also be re-used if you lid the
container to reduce evaporation.
HTH, email me if you're stuck.
Richard P. Grant MA DPhil
Structural Studies Group, MRC-LMB
Please reply to rpg 'at' mrc-lmb.cam.ac.uk
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