Colloidal Blue

Hiranya S. Roychowdhury hroychow at nmsu.edu
Mon Mar 13 12:01:57 EST 2000


CBB-G is the dye used inh Bradford's prot. assay. For staining gels, it uses
a slightly different solvent system, but I can't remember off the top of my hat.
CBB-G also stains the bands a little towards the "red" side, instead of the
usual blue coloration as seen with CBB-R.
Some say that CBB-G is more sensitive, but I used it a couple of times quite
a while back and found it to be not so. 

At 12:18 PM 3/13/00 +0000, Richard P. Grant wrote:
>In article <109ec386.d1a7c612 at usw-ex0109-066.remarq.com>, David Coulson 
><d.coulsonNOd.SPAM at qub.ac.uk.invalid> wrote:
>
>> I was wondering if anybody could help me with a "recipe" for
>> making up Collodial Blue (Comassie G-250) solution for
>> staining SDS-PAGE gels.  Do you just make it up in water?,
>> and if so what sort of concentration?
>
>Coomassie brilliant blue R250?  There is something which sounds similar 
>but doesn't work.  Can't remember what it is, exactly.
>
>Anyhoo:  0.1 % coomassie in 40% methanol, 10 % glacial acetic acid in 
>water (e.g. 2g dye, 800 ml MeOH, 200 ml GAA, make up to 2 l).  Store at 
>4C and can be reused several times.  Stain for about 15 - 20 mins at RT.  
>For destaining, I pour off the dye, then wash in dH20 (we have that on 
>tap) twice, then wash in destain (same as above, minus the dye) with 
>foam bungs to soak up the blue.  This can also be re-used if you lid the 
>container to reduce evaporation.
>
>HTH, email me if you're stuck.
>
>R
>
>-- 
>Richard P. Grant MA DPhil          
>Structural Studies Group, MRC-LMB
>http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
>Please reply to rpg 'at' mrc-lmb.cam.ac.uk
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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