Thrombin and high salt

Cyril Privenzentzev cprivenz at igr.fr
Tue Mar 14 06:15:01 EST 2000


Dear Netters:
I'd like to ask does high salt (up to 1M of NaCl) affect thrombin
protease activity? I need to cleave GST-fusion protein eluted in 0.9M
NaCl, 25 mM HEPES pH=7.6, 1 mM EDTA, 0.5 mM PMSF, 1 mM DTT, 10%
glycerol. I have to get rid EDTA and PMSF, it's clear, but what about of
salt and glycerol? May be someone know optimal condition for cleavage?
Thank you in advance,

Cyril V. Privezentz
CNRS UMR 8532-IGR
Villejuif
FRANCE  

TEl. +33 1 42115404
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