help wanted in solving a proteome problem

Chris LaRosa clarosa at biocomp.unl.edu
Tue Mar 14 16:47:05 EST 2000



sachin_16 at my-deja.com wrote:

> Hello reader
> I am stuck with a following problem
> I am running 2D gels but the proteins seem to get
> concentrated on acidic side of the gel.Tried
> changing the reagent but nothing seem to be

You need to supply alot more information about your ief system and
protocol in order for anyone to help you.    You may be doing a number
of things wrong.   Try contacting tech support for the company that
makes your ief apparatus, is a good cheap way to go.





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