inclusion bodies

xavier msxavierNOmsSPAM at unb.br.invalid
Wed Mar 15 06:10:49 EST 2000


I'm student and I'd like to know if there is another better
way to calculating the quantity of fusion protein from
inclusion bodies expressed in E. coli. Is the 55% of total
proteins described in literature a good % to consider? I
have been using this equation:

Ptn fusion(g) = dry weight(g/L) x 55% x %densitometry x
vol(L).

thanks,

Xavier


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