inclusion bodies

[xavier]

I'm student and I'd like to know if there is another better
way to calculating the quantity of fusion protein from
inclusion bodies expressed in E. coli. Is the 55% of total
proteins described in literature a good % to consider? I
have been using this equation:

Ptn fusion(g) = dry weight(g/L) x 55% x %densitometry x
vol(L).

thanks,

Xavier


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