Ultracentrifugation Problems

Markus Klein klein at biozentrum.uni-wuerzburg.de
Tue Mar 21 04:26:33 EST 2000


Hallo!

I´m desperately trying to ultracentrifugate a Buffer-Solution containing
4 M Guanidinium-Hydrochloride and 100 mM Tris-HCl, pH 8 at 100000g.
The Beckman Polycarbonat-Tubes  are not stable at this pH, so Beckman
told me to use Polyallomer-Tubes (for JA20 Rotors in Cooling
Centrifuge).  I could use them with an adapter in our 45Ti-Rotor. But
when I tried this, the tubes were deformed and leaked. QuickSeal are to
small for my volumes (about 200 to 500 ml) and a little bit expensive,
too.
Beckman is now telling me, that there is no known solution for my
problem.
I can´t believe, that is anybody else trying to ultracentrifuge a pH 8
solution.






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