post trans modification question

nospam at our.site nospam at our.site
Wed Mar 22 11:34:58 EST 2000


This message has been posted by:  612 right <i.hinners at REMOVE-THIS-TO-SENDicrf.icnet.uk>

Hello Dave,
I think it almost purely depends on the protein and its modification.
Glycosylation often results in a smear. I am working with a protein which migrates
faster after myristoylation, but other proteins would migrate slower. I believe
phosphorylation usually descreases mobility.... and so on....
So there is no strict rule or even a rule to calculate the mobility,
hope this helps, Ina


"D.D. Morgan" wrote:

> How does modification of a protein alter its apparent mwt on SDS-PAGE ?
>
> Is it purely the increase in mwt due to the added residues or do they have
> an effect on the overall charge of the protein altering its mobility ?
>
> Any answers gratefully recieved and are there any basic references for a
> "DNA man" to check out
>
> Thanks
>
> Dave Morgan
>
> --
>
> *******************************************************************************
> * Dave Morgan             | **.           .***.   .***.           .***.   .** *
> * Dept of Human Genetics  | | | *       * | | | * | | | *       * | | | * | | *
> * University of Newcastle | | | | *   * | | | *   * | | | *   * | | | *   * | *
> * 19/20 Claremont Place   | * | | | * | | | *       * | | | * | | | *       * *
> * Newcastle-Upon-Tyne     |   '***'   '***'           '***'   '***'           *
> * NE2 4AA                 |                                                   *
> * Tel: 0191-222-5129      |         e-mail: D.D.Morgan at newcastle.ac.uk        *
> * Fax: 0191-222-7143      |                                                   *
> *******************************************************************************

--
Ina Hinners
ICRF
Secretory Pathways Laboratory
44 Lincolns Inn Fields
WC2A 3PX London
UK
email: I.Hinners at icrf.icnet.uk






More information about the Proteins mailing list