resolution on PAGE??

Tadeusz Moczon moczon at twarda.pan.pl
Wed Mar 22 12:56:46 EST 2000


Ben Gantner wrote:
> 
> Hi all,
> 
> Does anyone have protocols or good web sites that discuss methods for
> improving resolution of SDS-PAGE?  I'm trying to get separation of a
> fairly complex mixture, and I'm not getting particularly good
> resolution.
> 
> Along the same lines, are there opinions out there on PDA vs. bis as
> x-linkers?
> 
> Thanks for the help,
> Ben
=============================
First, try to separate your samples in a linear concentration gradient
gel. If the sieving effect would not satisfy you, try to become
proficient in the 2D technique:
http://www.uct.ac.za/microbiology/manualin.htm

Good luck!
T.M.




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