Ultracentrifugation Problems

karlster karlster at excite.com
Wed Mar 22 13:23:52 EST 2000


In article <38D74048.68834890 at biozentrum.uni-wuerzburg.de>, Markus Klein
<klein at biozentrum.uni-wuerzburg.de> wrote:

> Hallo!
> 
> I´m desperately trying to ultracentrifugate a Buffer-Solution containing
> 4 M Guanidinium-Hydrochloride and 100 mM Tris-HCl, pH 8 at 100000g.
> The Beckman Polycarbonat-Tubes  are not stable at this pH, so Beckman
> told me to use Polyallomer-Tubes (for JA20 Rotors in Cooling
> Centrifuge).  I could use them with an adapter in our 45Ti-Rotor. But
> when I tried this, the tubes were deformed and leaked. QuickSeal are to
> small for my volumes (about 200 to 500 ml) and a little bit expensive,
> too.
> Beckman is now telling me, that there is no known solution for my
> problem.
> I can´t believe, that is anybody else trying to ultracentrifuge a pH 8
> solution.

Only suggestion I can make is kick over to a swinging bucket rotor like an
SW28 and use either polyallomer or UltraClear tubes. Capacity of rotor is
6X 39-40 ml which would accomodate your lower volume estimate (ie. 200
ml).

karlster




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