resolution on PAGE??

Richard J. Dudley rdudley at pitt.edu
Wed Mar 22 16:01:35 EST 2000


There are also different acrylamide mixes.  Amresco has a PAGE-Plus,
which has worked very well in my hands for extending the range of
separation, and for the small proteins (~ 4kDa)..  FMC's Long-Ranger is a
similar substance.  You might also want to investigate Tricine SDS-PAGE.
There are a couple of methods you can find in PubMed.  Novex's web site
(which I guess is now www.invitrogen.com) has some products and
references you also might find useful.

Good luck!

rich

Ben Gantner wrote:

> Hi all,
>
> Does anyone have protocols or good web sites that discuss methods for
> improving resolution of SDS-PAGE?  I'm trying to get separation of a
> fairly complex mixture, and I'm not getting particularly good
> resolution.
>
> Along the same lines, are there opinions out there on PDA vs. bis as
> x-linkers?
>
> Thanks for the help,
> Ben

--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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