Purification with His-Tag

Anton Tutter atutter at aim.salk.edu
Fri Mar 24 01:32:03 EST 2000

>> Can I remove the Imidazol just by dialyzing?
> Yes, we usually do that.

just a side warning about dialyzing the imidazole away from his-tag
proteins--his tagged proteins have a tendency to aggregate and precipitate
out of solution during the dialysis step.  this has been an ongoing topic of
discussion on this newsgroup.


More information about the Proteins mailing list