Purification with His-Tag

Anton Tutter atutter at aim.salk.edu
Fri Mar 24 01:32:03 EST 2000


>> Can I remove the Imidazol just by dialyzing?
> 
> Yes, we usually do that.


just a side warning about dialyzing the imidazole away from his-tag
proteins--his tagged proteins have a tendency to aggregate and precipitate
out of solution during the dialysis step.  this has been an ongoing topic of
discussion on this newsgroup.

--anton





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