Purification with His-Tag

Frank Fuerst fant.1 at gmx.net
Fri Mar 24 04:22:02 EST 2000


Anton Tutter schrieb:
> 
> >> Can I remove the Imidazol just by dialyzing?
> >
> > Yes, we usually do that.
> 
> just a side warning about dialyzing the imidazole away from his-tag
> proteins--his tagged proteins have a tendency to aggregate and precipitate
> out of solution during the dialysis step.  this has been an ongoing topic of
> discussion on this newsgroup.

And what would you recommend instead? Are there other possibilities to
get rid of imidazole, where the aggregation problem is minimized?

Frank
-- 
Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
ausrufender sondern ausufernder. [Michael Bauer in dnq]




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