Purification with His-Tag

Frank Fuerst fant.1 at gmx.net
Fri Mar 24 04:22:02 EST 2000

Anton Tutter schrieb:
> >> Can I remove the Imidazol just by dialyzing?
> >
> > Yes, we usually do that.
> just a side warning about dialyzing the imidazole away from his-tag
> proteins--his tagged proteins have a tendency to aggregate and precipitate
> out of solution during the dialysis step.  this has been an ongoing topic of
> discussion on this newsgroup.

And what would you recommend instead? Are there other possibilities to
get rid of imidazole, where the aggregation problem is minimized?

Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
ausrufender sondern ausufernder. [Michael Bauer in dnq]

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