Purification with His-Tag

Edith djianaNOdjSPAM at hotmail.com.invalid
Tue Mar 28 09:56:34 EST 2000


Hi sigrid,
you could try to eliminate most of the imidazole by
ultrafiltration (using the Amicon system). Step dilution
and ultrafiltration to decrease the imidazole concentration
to a limit where your protein will still be soluble.
Hope this can help you out.

Dr DJIANA
IRBV
University of Montreal


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