Purification with His-Tag

Edith djianaNOdjSPAM at hotmail.com.invalid
Tue Mar 28 10:09:01 EST 2000


I guess your protein has been cloned and expressed prior to
that purification.
I also used the His-Tag system to purify a cabbage methyl
transferase which has been cloned and expressed in E. coli.
But I could not detect any traces enzyme activity. I was
wondering what can be done to detect or to restore activity
(although bearing in mind that there could be a lack of
post-translational modification of the recombinant protein)?
Thanks in advance


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