Purification with His-Tag

ChenHA hzhen at freeuk.com
Tue Mar 28 17:28:29 EST 2000



Anton Tutter wrote:
> 
> >> Can I remove the Imidazol just by dialyzing?
> >
> > Yes, we usually do that.
> 
> just a side warning about dialyzing the imidazole away from his-tag
> proteins--his tagged proteins have a tendency to aggregate and precipitate
> out of solution during the dialysis step.  this has been an ongoing topic of
> discussion on this newsgroup.
>

I supposed as an occasional visitor to this newsgroup I must
have missed those discussions.  Could you provide more
information?  It is my understanding that it is in fact the
reverse, i.e. the presence of the His-tag helps to prevent
aggregation.  Are there specific examples of comparison of
propensity for aggregation by His-tagged and non-His-tagged
proteins?

> --anton




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