Purification with His-Tag
hzhen at freeuk.com
Tue Mar 28 17:28:29 EST 2000
Anton Tutter wrote:
> >> Can I remove the Imidazol just by dialyzing?
> > Yes, we usually do that.
> just a side warning about dialyzing the imidazole away from his-tag
> proteins--his tagged proteins have a tendency to aggregate and precipitate
> out of solution during the dialysis step. this has been an ongoing topic of
> discussion on this newsgroup.
I supposed as an occasional visitor to this newsgroup I must
have missed those discussions. Could you provide more
information? It is my understanding that it is in fact the
reverse, i.e. the presence of the His-tag helps to prevent
aggregation. Are there specific examples of comparison of
propensity for aggregation by His-tagged and non-His-tagged
More information about the Proteins