I guess this has been asked before, but as an old hand at protein purification but new to inclusion bodies, what do people find the best way to renature from 6M urea? Cheers, R -- Richard P. Grant MA DPhil Structural Studies Group, MRC-LMB http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html Please reply to rpg 'at' mrc-lmb.cam.ac.uk