help wanted in solving a proteome problem

sachin_16 at my-deja.com sachin_16 at my-deja.com
Wed Mar 29 09:58:47 EST 2000


Thanx chris
The one that I am uysing is from Pharmacia
I am using dehydrated IEF gels which are rehydrated in solution(it has
CHAPS biolyte3-10urea andDTE.The samples were made in PGSK(phosphate
buffer and glucose refer to the EXPASY book protein extraction
fromYeast)
The ief run was as follows 300V for3 hrs followed by 3500V for 24 hrs

Waiting for more suggestion
Sachin


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