largest protein structure through NMR

John Kuszewski johnk at speck.niddk.nih.gov
Thu Mar 30 14:57:12 EST 2000


Hi,

The largest our lab (Clore/Gronenborn) has done is pdb code 3EZE, a ~40
kDa
protein/protein complex, and pdb 2EZO, a 44 kDa protein trimer.  

That's about as big as it gets right now.

Kurt Wuthrich has reported spectra of a 110 kDa system, but hasn't
solved
a structure.  His is a special case, because it's something like a
10-mer,
so the number of peaks is only as great as an 11 kDa protein, and each 
individual peak's height is also multiplied by 10.



Debashis Mukhopadhyay wrote:
> 
> Hi all,
> can anybody tell me which is the largest protein structure (and its size)
> solved so far through an NMR experiment? Or what is the average solvable
> size, in general?
> Thanx in advance,
> Deb
> 
> ------------------------------------------------------------------------------
>   ("`-''-/").___..--''"`-._
>    `6_ 6  )   `-.  (     ).`-.__.`)          Debashis Mukhopadhyay
>    (_Y_.)'  ._   )  `._ `. ``-..-'           Sr. Research Fellow, C&MB Div.,
>  _..`--'_..-_/  /--'_.' ,'                   Saha Institute Of Nuclear Physics,
> (il).-''  (li).'  ((!.-'                     1/AF, Bidhannagar,Calcutta-700 064
> Tel# +91-033-3375345 (X:308) | Fax# +91-033-3374637 |   deb at cmb2.saha.ernet.in
> ------------------------------------------------------------------------------
> 
> ---

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