Guanidium unfolding

Radha Chauhan radha at imtech.ernet.in
Tue May 9 09:15:44 EST 2000


Hi all!

I am a novice with protein unfolding problems and am facing a strange
problem:

I wanted to unfold my protein with guanidium chloride.  As a test, I
added some protein (25 ug/ml) to 4 M guanidium in 20 mM tris ph 7.5.
When I meaured fluorescence, the native protein gave a good signal, so
did a small signal for the blank (buffer + 4 M guanidium).

However, on with the test (protein+ 4 M guanidium), I got a perfect
straight line at 0 intensity units. I wonder what's wrong?  Any
suggestions?

Thanks in advance for all help,

Bhupesh. 9-5-00.

******************************************
*                                        *
* Bhupesh Taneja, SRF (Graduate student) *
*   Institute of Microbial Technology,   *
*   Sector 39-A,                         *
*   Chandigarh - 160 036. (INDIA).       *
* Ph: +91 172 695226 extn 494            *
*                                        *
* Email : bhupesh at lion.imtech.ernet.in   *
*         bhupesht at yahoo.com             *
 http://www.bragg.imtech.ernet.in/bhupesh
*                                        *
******************************************

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