Deglycosylation

James Fethiere james at mpimf-heidelberg.mpg.de
Wed May 10 04:28:01 EST 2000


Somebody on this list once suggested to me to increase NaCl up to 0.7 M
when deglycosylating with EndoF in order to avoid precipitation of my
glycoprotein.  I would like to know if Sialidases are also resistant to
the high salt concentration.

P.S.  I am using a combination of EndoF2 and  Sialidase C in 250 mM Na
Acetate buffer pH 5

Thank you

James

--
************************************************************************
* Dr. James Fethiere                                                   *
* MPI-Medical Research            Int'l Tel: +49-6221-486154           *
* Ion Channel Structure Group     Int'l Fax: +49-6221-486437           *
* Jahnstrasse 29                  email: james at mpimf-heidelberg.mpg.de *
* 69120, Heidelberg                                                    *
* Germany                                                              *
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