Stripping nitrocellulose membranes containing phosphoprotein

Gary gkrause at med.wayne.edu.spam
Wed May 10 12:06:01 EST 2000


I'm trying to strip a nitrocellulose membrane [10 mM b-mercaptoethanol, 0.1%
SDS, 20 mM Tris, pH 6.5] to restain it with another antibody .  The signals
I 'm looking for are phosphoproteins. I appear to be losing more signal than
I would expect.  Is there a chance that I'm hydrolyzing the phosphate group
during stripping?  How do I avoid this?  Thanks.

Gary








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