blue native PAGE and molecular mass analisys

ChenHA hzhen at freeuk.com
Fri May 12 05:58:44 EST 2000


Frank Fuerst wrote:
> 
> It is also possible that the kinetics of association and dissociation
> is faster than the retention time - then you'll never see dimeric or
> tetrameric peaks. This is because one molecule is and runs as a dimer
> for a fraction (depending on the equilibrium constant) of the time,
> and a tetramer for the rest. If dimers and tetramers each account for
> about half of the protein, you'll see a broad peak with the retention
> time of a trimer...
> 

That was my first reaction when I read that it came out a
single peak.  It may be possible to change the equilibrium
by changing the pH and/or buffer composition.  Very often
this kind of rapid equilibrium is mediated by charged
species, therefore changing the pH can shift the
equilibrium. There are a number of papers on that, so do a
paper search. 

> Yours, Frank
> --
> Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
> ausrufender sondern ausufernder. [Michael Bauer in dnq]




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