SDS page - the images

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed May 17 13:58:08 EST 2000


In article <8fuo11$1ftm at r02n01.cac.psu.edu>,
  "Kunal Mukhopadhyay" <kunalm at iname.com> wrote:
> Here are the images of the gels that I mentioned in my earlier post.
>
> www.personal.psu.edu/kxm66/gels/tiffa1.jpg
>
> www.personal.psu.edu/kxm66/gels/tiffa2.jpg
>
> They certainly are nothing to brag about! Any comments would be appreciated.
>
> P.S Yes...I have run better ones, just in case anyone may wonder.
>

My first guess is that you have an air bubble trapped on the bottom
plate, disrupting the current flow around that region of the gel.

What kind of a gel setup do you use? The older ones that formed the
bottom by putting a spacer inside the plates were prone to this. In the
"olden days" we used to keep either a syringe with a bent needle or a
"bent" pasteur pipet around so that we could squirt buffer up into that
space after putting the gel in the tank in order to flush the bubbles
away.

Nick

--
_______________________________________________
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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