SDS page - the images

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed May 17 16:16:21 EST 2000


In article <200005172044.OAA295134 at nestor.NMSU.Edu>,
  hroychow at nmsu.edu ("Hiranya S. Roychowdhury") wrote:
> I wonder!
> A few air bubbles would certainly distort the run, but the picture is like
> nothing I have seen before (and I have more than a little experience in
> SDS-PAGE). Is it possible that somehow the gel is polymerizing at
> significantly diffrent rate in the middle? More appropriately, the the gel
> seems to be of a higher %-age (hence the retardation) where the stained band
> is "frowning" so much.
>

Kunal:

Did the dye front also migrate abnormally, or just the proteins?

N

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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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