SDS Page

Scott Johnson sjohnson at inetnebr.com
Thu May 18 00:39:34 EST 2000


Kunal,
    Does this occur when you use a certain gel box?  Are there air bubbles
trapped at the bottom of the gel?  Air bubbles can disrupt the current flow.
What are the nature of the samples in the lanes with these "squirms"?  High
salt samples can result in strange migration patterns such as these.  Also,
maybe if this occurs in the same gel box, try replacing the platinum wiring,
or at least cleaning them.
Hope this helps,

Good luck!

Kunal Mukhopadhyay <kunalm at iname.com> wrote in message
news:8fuhan$17ie at r02n01.cac.psu.edu...
> Hello again fellow scientists
> A note of gratitude to all who replied to my earlier query on BamHI. I was
> pleased to see that it worked and now am awaiting the confirmation
(sequence
> report).
> In the meanwhile I had another query :
>
> While running SDS Page gels for my proteins, I find occasionally a
"squirm"
> towards the end of the gel totally ruining the gel. I thought it might be
> due to the heat generated during the electrophoresis. However when running
> two gels parallel on different units, one ends up with Squirms, the other
> doesn't.
>
> Does anyone face similar problems? Is there a way to minimize the effect?
>
> I'll try to put up a picture of the gel if I can on the web.
>
> Thanks
> Kunal
>
> --
>
>
>
****************************************************************************
> ******
> What does it profit a man if he gains the world... but loses his soul?
>
****************************************************************************
> ******
>
> Kunal Mukhopadhyay
>
> 52, 152 Davey Lab,
> Department Of Chemistry
> Pennsylavania State University,
> State College, PA 16802
> USA
>
> (814) 865-1273
>
> email : kunalm at psu.edu
>
>
>
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> >>
>
>






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