GST dimerization

Peter Drueckes drueckes at tumorbio.uni-freiburg.de
Mon May 22 08:33:54 EST 2000



Hi everybody,

GST or the GST-domains of fusion proteins are known to dimerize. I
wonder if anybody might know of conditions that avoid this dimerization
in order to have a defined size of the protein. This would help to
separate the GST from other proteins by gel filtration e.g. after
thrombin cleavege of the fusion protein.

Alternativly conditions where the dimerization is quantitative could
also work.

All suggestions are wellcome.
Thanks, Peter.





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