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Pathos pathos at
Sat May 27 11:05:07 EST 2000

In article <3929662D.5510EBA3 at>, Savita Shah
<spshah at> wrote:

> (I normally ppt proteins with 10% TCA, wash with
> 80% Acetone,

This is sometimes a bad idea.  TCA is a potent dehydrating substace. 
Proteins that are hydrophilic may not go back into solution following TCA
precipitation.  I prefer to do phenol:ether extraction and lyophilize the


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