protein extraction from leaves

Savita Shah spshah at stanford.edu
Tue May 30 19:46:24 EST 2000


In addition to Thiourea at 5mM use sodium metabisulphite (50mM or 100mM), PVPP 1% and beta mercaptoethanol (optional) 0.5%..

Good Luck

Savita

Gerardo Perez Quimica UNALCOL wrote:

> Hello Ligia:
> There is a procedure , which have worked well in our hands, described by :
> Cremer F., van de Walle C. in Anal Biochem . 147 : 22-26, 1985.
>
> As polyphenols and other oxidable pigments are a nuisance we use thiourea
> to reduce the extent of oxidation. Have a look at :
>  Van Driessche et al ., Anal Biochem., 141:184-188, 1984
>  Good luck
>
> Gerardo Perez
> ;
>
> On Mon, 22 May 2000, llebron wrote:
>
> > Hi there:
> > I worrk with coffee and the pathogen fungi Cercospora
> > coffeicola.  I wonder to extract pathogenesis related
> > proteins from coffee leaves. Do anyone know a good protocol
> > for the extraction of leaves's proteins
> > Thanks in advance
> > Ligia
> >
> >
> > * Sent from AltaVista http://www.altavista.com Where you can also find related Web Pages, Images, Audios, Videos, News, and Shopping.  Smart is Beautiful
> >
>
> ---






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