Soluble scFv's

Richard Buick Richard.Buick at
Fri Nov 10 19:18:35 EST 2000

At present I am trying to express scFv's in the pRSET and pET systems. The
trouble is expression is too efficient and the scFv's are produced as
insoluble inclusion bodies.  Is there any simple answer that I'm missing?
Re-folding attempts have been unsuccessful. I'm now trying to express in
salt-inducible BL21's.
Any suggestions?


Richard J. Buick

E-mail: mail at
Tel(GSM): +44 7801 416792


More information about the Proteins mailing list