Soluble scFv's

Richard Buick Richard.Buick at btinternet.com
Fri Nov 10 19:18:35 EST 2000


At present I am trying to express scFv's in the pRSET and pET systems. The
trouble is expression is too efficient and the scFv's are produced as
insoluble inclusion bodies.  Is there any simple answer that I'm missing?
Re-folding attempts have been unsuccessful. I'm now trying to express in
salt-inducible BL21's.
Any suggestions?

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Richard J. Buick

E-mail: mail at richardbuick.com
URL: http://www.richardbuick.com
Tel(GSM): +44 7801 416792

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