Soluble scFv's

baltim baltim1 at home.com
Fri Nov 10 21:31:24 EST 2000


I've had increased solubility by expressing with the pET system in
BL21 @ 20 C. Frequently those that are completely insoluble @ 37 will
be 50% or more soluble @ 20.


On Sat, 11 Nov 2000 00:18:35 -0000, "Richard Buick"
<Richard.Buick at btinternet.com> wrote:

>At present I am trying to express scFv's in the pRSET and pET systems. The
>trouble is expression is too efficient and the scFv's are produced as
>insoluble inclusion bodies.  Is there any simple answer that I'm missing?
>Re-folding attempts have been unsuccessful. I'm now trying to express in
>salt-inducible BL21's.
>Any suggestions?
>
>--
>
>
>
>***************************************************
>Richard J. Buick
>
>E-mail: mail at richardbuick.com
>URL: http://www.richardbuick.com
>Tel(GSM): +44 7801 416792
>
>***************************************************
>
>






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