Help with signal sequences/peptidases

Sarah Rogers sarah.rogers at bbsrc.ac.uk
Mon Nov 13 05:33:32 EST 2000


I would like to express a type II membrane protein that I am working on
in a mammalian cell line, and need to include a signal sequence to
direct the protein to the media for purification.  I have the sequence
for the mouse CD8 signal sequence, but I am unsure as to how much of the
sequence I need to include for correct targeting of the protein and
subsequent cleavage of the signal.  

Any help or advice would be greatly appreciated!

Sally Rogers (Ph.D. student)


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