8M urea and 6M Gu-HCl: can they lysis yeast cell wall

clarosa clarosa at biocomp.unl.edu
Wed Nov 29 12:50:55 EST 2000


Think about an additional  mechanical means to disrupt such as a bead
beater, sonication,  pressure disruption.  I do not believe those agents
break the cell wall.  Surely there are well worked out protocols for yeast
protein extraction.

Dagmawi Iyasu wrote:

> Hello ...
> I have used both 8M urea and 6M Gu-HCl to lyse E. coli to purify my
> protein of interest.  I just started to work with yeast and I was
> woundering if I can use them for that purpose also. Are they strong
> enough to bust the cell wall open? I would like to avoid SDS in the
> lysis buffer; that is why I would like to use them if I can. I thank you
> in advance for the help.
>
> Mawi
>
> ---






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