easy elution from acrylamid gel?
mw132 at mole.bio.cam.ac.uk
Thu Nov 30 08:35:29 EST 2000
Once I cut a really big band from a gel, cut it up and left it in an
eppendorf with a very small amount of buffer at four degrees. Next
morning I ran a gel of the buffer and found that protein had diffused out
of the gel. Mike.
On Thu, 30 Nov 2000, M. Hertel wrote:
> we are looking for a low tech, handmade method for eluting a Protein out
> of an acrylamid gel band. Nothing where You need an electroeluter or
> columns etc. but something simple like dialysis, elution in a agarosegel
> chamber , boiling in a buffer???
> thanks for Ideas
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