protein precipitation

Michael Witty mw132 at mole.bio.cam.ac.uk
Sun Oct 1 15:56:42 EST 2000


Dear Emir, thanks for the tip.  My supervisor suggested a similar thing to
me BUT when I tried it out with a plain wate solution I found a lot of PEG
penetrated the membrante (mwc 12000).
     Do you think the membrane is not really mwc 12000 or
     my PEG is not really as high molecular weight as I thought

     OR should I not really worry about getting PEG mixed up with my
     protein.

Mike.

On Fri, 29 Sep 2000, Emir Khatipov wrote:

> Just an old method for concentrating protein solutions: pour your solution
> into dialysis tube and dig the tube into the beaker with dry PEG 20,000. PEG
> will absorb the liquid really fast (2-3-hrs).






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