protein precipitation

Peeter Toomik peeter.toomik at ut.ee
Tue Oct 3 00:59:20 EST 2000


You can use dry Sephadex instead of PEG.
Peeter Toomik

Michael Witty wrote:
> 
> Dear Emir, thanks for the tip.  My supervisor suggested a similar thing to
> me BUT when I tried it out with a plain wate solution I found a lot of PEG
> penetrated the membrante (mwc 12000).
>      Do you think the membrane is not really mwc 12000 or
>      my PEG is not really as high molecular weight as I thought
> 
>      OR should I not really worry about getting PEG mixed up with my
>      protein.
> 
> Mike.
> 
> On Fri, 29 Sep 2000, Emir Khatipov wrote:
> 
> > Just an old method for concentrating protein solutions: pour your solution
> > into dialysis tube and dig the tube into the beaker with dry PEG 20,000. PEG
> > will absorb the liquid really fast (2-3-hrs).






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