peeter.toomik at ut.ee
Tue Oct 3 00:59:20 EST 2000
You can use dry Sephadex instead of PEG.
Michael Witty wrote:
> Dear Emir, thanks for the tip. My supervisor suggested a similar thing to
> me BUT when I tried it out with a plain wate solution I found a lot of PEG
> penetrated the membrante (mwc 12000).
> Do you think the membrane is not really mwc 12000 or
> my PEG is not really as high molecular weight as I thought
> OR should I not really worry about getting PEG mixed up with my
> On Fri, 29 Sep 2000, Emir Khatipov wrote:
> > Just an old method for concentrating protein solutions: pour your solution
> > into dialysis tube and dig the tube into the beaker with dry PEG 20,000. PEG
> > will absorb the liquid really fast (2-3-hrs).
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