2-D PAGE set-up question
Paul S. Brookes.
brookes at uab.edu
Tue Oct 10 13:25:08 EST 2000
We've used the Pharmacia multiphor system without much trouble. It is
expensive if you use the pre-made strips though.
Our main problem appears to be in getting the strips loaded into the gel to
run the 2nd dimension. (we currently use Biorad mini-gels). Even with
super-heated agarose we often end up with air bubbles around the
strip. If any manufacturer has developed a system to streamline this
process, I'd say go with that one.
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
The quality of e-mails can go down as well as up
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Proteins