2-D PAGE set-up question

Paul S. Brookes. brookes at uab.edu
Tue Oct 10 13:25:08 EST 2000


We've used the Pharmacia multiphor system without much trouble.  It is 
expensive if you use the pre-made strips though.

Our main problem appears to be in getting the strips loaded into the gel to 
run the 2nd dimension.  (we currently use Biorad mini-gels).  Even with 
super-heated agarose we often end up with air bubbles around the 
strip.   If any manufacturer has developed a system to streamline this 
process, I'd say go with that one.

Regards
PSB

_________________________________________
Dr. Paul S. Brookes.            (brookes at uab.edu)
UAB Department of Pathology,   G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915     Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes

The quality of e-mails can go down as well as up
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