hydroxyapatite

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Oct 11 07:56:07 EST 2000


I tried making my own hydroxyapatite once, using a calcium salt and
phosphate salt to make a precipitate (I forget which salts I used but
could look them up if anyone is interested).  BUT when I added a protein
extract (E. coli crude extract) nothing would stick to the column!  Is
there some kind of secret to making hydroxyapatite (other than buying it
from Bio Rad, as I do now).  Mike

On Tue, 10 Oct 2000, Julius wrote:

> I allways use Calbiochem hydroxyapatite HR and have no problems.
> regards.
> 
> Jeremy Murray <jez at csb.yale.edu> wrote in message
> news:39E1136F.A5B3DA76 at csb.yale.edu...
> > hey
> > I am looking for suppliers of hydroxyapatite for chromatographic
> > purposes. I am currently using a CHT-5 column purchased from
> > BioRad, the media in the column has taken a recent battering and
> > needs to be renewed. I have contacted BioRad about this but they
> > are unable to supply me with the required quantity within a reasonable
> > period of time. I would be grateful if anyone could recommend the names
> > of suppliers that they have used and any suggest protocols to prepare
> > the
> > media for column chromatography.
> > tnx in advance. J.






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