hydroxyapatite

Jeremy Murray jez at csb.yale.edu
Wed Oct 11 21:40:32 EST 2000


i have just ordered some some from Sigma, $47 for 10 mL.
I am gonna do a comparison of the BioRad and Sigma varieties.
I will report back to the ng, if anyone is interested.
l8rz. J.

Michael Witty wrote:

> I tried making my own hydroxyapatite once, using a calcium salt and
> phosphate salt to make a precipitate (I forget which salts I used but
> could look them up if anyone is interested).  BUT when I added a protein
> extract (E. coli crude extract) nothing would stick to the column!  Is
> there some kind of secret to making hydroxyapatite (other than buying it
> from Bio Rad, as I do now).  Mike
>
> On Tue, 10 Oct 2000, Julius wrote:
>
> > I allways use Calbiochem hydroxyapatite HR and have no problems.
> > regards.
> >
> > Jeremy Murray <jez at csb.yale.edu> wrote in message
> > news:39E1136F.A5B3DA76 at csb.yale.edu...
> > > hey
> > > I am looking for suppliers of hydroxyapatite for chromatographic
> > > purposes. I am currently using a CHT-5 column purchased from
> > > BioRad, the media in the column has taken a recent battering and
> > > needs to be renewed. I have contacted BioRad about this but they
> > > are unable to supply me with the required quantity within a reasonable
> > > period of time. I would be grateful if anyone could recommend the names
> > > of suppliers that they have used and any suggest protocols to prepare
> > > the
> > > media for column chromatography.
> > > tnx in advance. J.

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