arsphys at cc.usu.edu
Mon Oct 16 10:57:44 EST 2000
Many proteins will dissolve more readily in base. You might try dissolving
the protein in 0.1 M NaOH and then neutralize it if necessary. Adding some
(0.1-0.15 M) NaCl may also help.
At 12:42 PM 10/16/2000 +0200, you wrote:
>I am a nutritionist trying to prepare a fibrinogen solution (2g/L).
>Problem is that it does not dissolve readily. It seems to stick
>together. I have now added the fibrinogen powder carefully to the
>phosphate buffered saline and just let it stay on top until it seems
>dissolved - this is also not working well. Slowly mixing it leads to
>aggregates, vigorous mixing is a disaster, ultra sound does not help,
>neither does warming the buffer. Should I try using something else
>rather than PBS (7.4)? Any suggestions will help
More information about the Proteins