Solubilising fibrinogen

Emir ekhatipo at NOSPAMmidway.uchicago.edu
Tue Oct 24 11:55:33 EST 2000


You may try 0.1M HCl as well and then neutralize if needed.

"netwerkid" <netwerkid at puknet.puk.ac.za> wrote in message
news:39EADB9C.3CE71FD8 at puknet.puk.ac.za...
> I am a nutritionist trying to prepare a fibrinogen solution (2g/L).
> Problem is that it does not dissolve readily.  It seems to stick
> together.  I have now added the fibrinogen powder carefully to the
> phosphate buffered saline and just let it stay on top until it seems
> dissolved - this is also not working well.  Slowly mixing it leads to
> aggregates, vigorous mixing is a disaster, ultra sound does not help,
> neither does warming the buffer.  Should I try using something else
> rather than PBS (7.4)?  Any suggestions will help
>
> Johann Jerling
>







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