Solubilising fibrinogen

[mvdplas at dds.nll]

On Mon, 16 Oct 2000 12:42:36 +0200, netwerkid
<netwerkid at puknet.puk.ac.za> wrote:

>I am a nutritionist trying to prepare a fibrinogen solution (2g/L).
[snip]
>neither does warming the buffer.  Should I try using something else
>rather than PBS (7.4)?  Any suggestions will help

Yes. PBS contains phosphate, and that will bind calcium present in
fibrinogen. Try tris-buffered saline or another positively charged
buffering agent.