pH-buffer system

Sigrid Van Boxstael svboxsta at vub.ac.be
Thu Oct 26 06:57:50 EST 2000


Hi,

I'd like to determine the activity of my protein in a whole pH-range
from 5 to 11.
I could use different buffers, like MES, CAPS, MOPS, CHES, Tris, ... who
all have a
buffering-capacity around their pKa.
If  I would use them all separately, I presume that there might be an
influence of the
type of buffer on my activity.
Does anyone now either it is adivisable to mix the buffers and adjust to
different pH's?
Does anyone have a proposal for a buffer system or maybey a reference?

Thanks,
Sigrid







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