Protease specificity

Jeremy Murray jez at csb.yale.edu
Fri Sep 1 20:49:30 EST 2000


I can recommend TEVP. There is a recombinant version
sold by GibcoBRL.
hth. j

Paul wrote:

> rwn wrote:
>
> > Hi there
> >
> > Does anyone have information regarding the specificity of
> > proteases. I am interested in comparing proteases normally
> > used for cleaving fusion proteins i.e. factor Xa and
> > thrombin.
> >
> >
>
> A few years ago I had problems with Factor Xa cleaving within
> one of my proteins as well as at the linker between it and MBP
> (from Biolabs' pMAL-c2).  I asked their tech support and was
> told that FXa will sometimes cleave at sites that resemble or contain
> a partial recognition sequence: i.e E/D-G-R or even G-R instead
> of the canonical I-E/D-G-R.  This made sense form the size of the
> unwanted fragments and sequence of my protein.  No references
> for this I'm afraid.
>
> Cheers
>
> Paul Digard

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