Aggregating protein

James Sandy james.sandy at pharm.ox.ac.uk
Mon Sep 11 06:26:47 EST 2000


Hi there, I have a problem. I am purifying arylamine n-acetyltransferase and
the prep is fine until the protein is being concentrated. Around 5 mg/ml the
protein seems to aggregate and fall out of solution. Does anybody know any
cunning methods to stop proteins falling out of solution when being
concentrated?

Thanks for any advice,


James Sandy
Research Assistant
University Dept. of Pharmacology
Oxford University
email james.sandy at pharm.ox.ac.uk







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