Aggregating protein

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Sep 11 07:12:20 EST 2000


Dear Sandy, what buffer are you using?  How about using a different pH
to avoid pI or some salts to improve solubility/stability.  Mike.

On Mon, 11 Sep 2000, James Sandy wrote:

> Hi there, I have a problem. I am purifying arylamine n-acetyltransferase and
> the prep is fine until the protein is being concentrated. Around 5 mg/ml the
> protein seems to aggregate and fall out of solution. Does anybody know any
> cunning methods to stop proteins falling out of solution when being
> concentrated?
> 
> Thanks for any advice,
> 
> 
> James Sandy
> Research Assistant
> University Dept. of Pharmacology
> Oxford University
> email james.sandy at pharm.ox.ac.uk
> 
> 
> 
> 






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