how to solve keratin
sap at gbf.de
Wed Sep 13 03:53:17 EST 2000
I try to solve keratin (hair of albino rats) to examine the proteins, I
m looking for a special one. But all I tried (reduction with DTT or
tributylphosphine and high temperature 95 °C) could not help me to solve
all the material. Every time a pellet is left.
So, I m looking for a reducing agent or methode to solve hair totally
and leave the proteins intact for 2 D electrophoresis.
thanks for ideas
Sandra (sap at gbf.de)
More information about the Proteins