how to solve keratin

sandra pilawa sap at gbf.de
Wed Sep 13 03:53:17 EST 2000


I try to solve keratin (hair of albino rats) to examine the proteins, I
m looking for a special one. But all I tried (reduction with DTT or
tributylphosphine and high temperature 95 °C) could not help me to solve
all the material. Every time a pellet is left.
So, I m looking for a reducing agent or methode to solve hair totally
and leave the proteins intact for 2 D electrophoresis.

thanks for ideas
Sandra (sap at gbf.de)






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